The chamber voted 24-23 against the ballot measure after the bill passed the House overwhelmingly.
The North Dakota Senate’s rejection of the resolution means residents of the nation’s least populated state may have to wait until at least 2024 before the legislature reconsiders a sports betting ballot measure.
Sports betting faced opposition from religious groups as well as North Dakota’s five gaming tribes, which earlier this year saw their own hopes of operating sports betting dashed when the House voted down a separate bill.
The Senate is still waiting to vote on an online poker ballot measure. The House voted in favor of the legislation, House Concurrent Resolution 3012, in a 54-40 vote.
The bill’s sponsor, Rep. Jim Kasper, R-Fargo, has tried but failed to pass an online poker constitutional amendment since 2005.
The Senate vote comes a week after South Dakota voted in favor of a retail-only sports betting bill after South Dakotans legalized sports wagering at the ballot box last November.
South Dakota has more than 100,000 residents than its northern neighbor. Voters there approved sports betting by a 58% to 42% margin.
